SCREENING METHODS: Antifungal assays(1)

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Antifungal assays(1)

Antifungal assays are regularly used to determine whether plants extracts will have potential to treat human fungal infections (e.g. Tinea) or have use in agricultural/horticultural applications. In general these assays are quick, low cost, and do not involve access to specialized equipments. Activity of plant extracts against the yeast Candida is typically assessed using the disk or well diffusion methods described above, and many studies report anti-candidal activity with antibacterial activity rather than with activity against fungi for this reason (Haraguchi et al, 1999; Rahua et al, 2000; Wilkinson and Cavanagh, 2005; Panda et al., 2010b,d,e,f).

Activity against filamentous fungi can be evaluated in well diffusion, agar dilution, and broth/microbroth methods with many of the same limitations and advantages as previously discussed for antibacterial assays (Inouye et al, 2001). When the well diffusion and disk diffusion techniques are used, fungal plugs are removed from an actively growing colony and placed at a predetermined distance (typically 2 cm) from the centre of an agar dish.

Representative APR 391%

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