SCREENING METHODS: Broth dilution methods(3)

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While these micro-broth methods generally work well for plant extracts, problems arise when the extract is heavily colored as this can interfere with the measurement of the indicator chemical. Further, as these methods use plastic microtiter plates, essential oils that have a solvent action on plastics (e.g. Letospermum petersonii, Backhousia citriodora) cannot be used. Also the addition of essential oils to media, changes its pH and this might be expected to be more significant in small volumes, like the micro broth method (Hood et al, 2004). Micro broth methods are also less time and resources intensive than other broth methods because bacterial count are eliminated.

With both the agar and broth dilution assays, the objective is to generate a single statistic to describe the inhibition of a microorganism at a specific endpoint in time. The measurement of inhibition at a specific time is termed the minimum inhibitory concentration (MIC). The MIC may be defined as the lowest concentration at which no growth occurs in a nutrient medium. However, the definition of the MIC differs between publications.

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