SCREENING METHODS: CONCLUSIONS(2)

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Attention to these issues could accompany in a poorly needed new era of chemotherapeutic treatment of infection by using plant derived principles. This review outline the main methods used in the evaluation of antimicrobial activity of plant extracts; each method has advantages and limitations and all have been widely cited in the literature. The question […]

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SCREENING METHODS: CONCLUSIONS(1)

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Scientists from divergent fields are investigating plants anew with an eye to their antimicrobial utility. All over the world thousands of phytochemicals have found which have inhibitory effects on all types of microorganisms in vitro. There is still a need for more scientific evaluation of Asian herbal medicines including their active constituents, synergistic interactions, formulation […]

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SCREENING METHODS: Confocal laser scanning microscopy(part 2)

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To study the antifungal activity through CLSM method the plant extract with MIC concentration will be prepared. Two days old fungal culture will be developed by culturing the fungal strains on PDA with the treatment of plant extract either by disc diffusion or agar cup method. Controls without the plant extract are also cultured. The […]

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SCREENING METHODS: Confocal laser scanning microscopy(part 1)

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After all these treatment finally the stained sections will be observed with a transmission electron microscope. Ultrathin sections obtained by conventional procedures, namely fixation with aldehydes, post-fixation with osmium tetraoxide, dehydratation and embedding in Epoxy resin, allow the observation of membrane and cytoplasmatic alterations. Treatment with AMPPs can induce several external and internal changes such […]

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SCREENING METHODS: Transmission electron microscopy(1)

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The specimens are then fixed with modified Karnovsky’s fixative, consisting of 2% (v/v) glutaraldehyde and 2% (v/v) paraformaldehyde in 0.05 M sodium cacodylate buffer solution (pH 7.2) at 4 °C for overnight. Subsequently, the fixed specimens are washed with the buffer solution three times for 10 min each. The specimens were then will be post-fixed […]

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SCREENING METHODS: Scanning electron microscopy(2)

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Once the sample is vapor fixed, the planchette will be plunged into liquid nitrogen -210 °C and then transferred on to the “peltier-cooled” stage of the freeze dryer, and freeze drying of the specimen will be kept for 10 h. Finally, the freeze dried specimen will be sputter coated with 5-10 nm gold before viewing […]

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SCREENING METHODS: Scanning electron microscopy(1)

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After treatment with plant extract, SEM observation will be carried out on fungal pathogens. The method involves the preparation of a Petridish containing 15-25 ml potato dextrose agar, seeded with 1 ml of the fungal conidial spore suspension at a known concentration (105 spores per ml). 1 ml of the extract at the concentration of […]

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