SCREENING METHODS: In situ antifungal activity

Comments 0

Dose relationships and mechanistic studies are some of the classical examples. However, the challenges with in vivo animal studies are also many, including what dose, route and for how long? Extrapolation of data from animals to humans and vice versa is difficult. Electron microscope has played an important role for visual inspection of fungal pathogens.

Comments »

SCREENING METHODS: Comparison between(2)

Comments 0

Additionally, de-differentiation and instability of cell lines adds to some uncertainties. Additional variables that affect analysis include variability in media composition, temperature, viscosity, osmolarity, and buffering. Naturally, therefore, in vitro bioassays cannot totally replace in vivo studies. However, the advantages of in vitro studies are many. Thus, we can study cell interactions, cell-environment interactions, intracellular […]

Comments »

SCREENING METHODS: Comparison between(1)

Comments 0

In vitro assays are useful while working with crude extracts. However, with herbal materials several issues come up. For example, what dose to use, what extract to use and what cut-offs to use? Doing viability assays (using Trypan Blue exclusion or MTT assays) help in deciding upper cut-offs, however, it is not fail-safe to lead […]

Comments »

SCREENING METHODS: Antibacterial activity

Comments 0

The best example of this is tea tree oil, which has been shown to have excellent activity in vitro against the fungi responsible for various tinea’s (MIC 0.004-0.06%) (Hammer et al, 2002) yet the results from clinical trials have been far from conclusive (Satchell et al, 2000). This illustrates the caution with which researchers should […]

Comments »

SCREENING METHODS: In Vivo assessment

Comments 0

The preceding discussion clearly demonstrates the similarity in methods used for in vitro antibacterial and antifungal assays of plant extracts and there are many papers in the literature using one of more of the methods. A smaller number of research groups have moved beyond the in vitro environment and are investigating the in vivo efficacy […]

Comments »

SCREENING METHODS: Inoculum

Comments 0

Mueller-Hinton medium allows good growth of most non-fastidious bacteria and is generally low in antagonists. It also meets the requirements of the NCCLS standard and is recommended as reference medium for agar and broth-dilution tests (Anon, 2003). The level of infection, i.e. inoculum concentration can have a profound influence on the antibacterial and antifungal potency […]

Comments »

SCREENING METHODS: Growth medium

Comments 0

Although many bacteria grow in a free-living, ‘planktonic’ state, it is quite common for them to adhere to surfaces by producing extracellular polymeric substances (EPS), e.g. biofilms. Due to their higher resistance against antimicrobial agents, an interesting option in antibacterial research is to include a bacterial biofilm model (e.g. Staphylococcus aureus ATCC6538).

Comments »
Pages: Prev 1 2 3 4 5 6 7 8 9 10 Next

Representative APR 391%

Let's say you want to borrow $100 for two week. Lender can charge you $15 for borrowing $100 for two weeks. You will need to return $115 to the lender at the end of 2 weeks. The cost of the $100 loan is a $15 finance charge and an annual percentage rate of 391 percent. If you decide to roll over the loan for another two weeks, lender can charge you another $15. If you roll-over the loan three times, the finance charge would climb to $60 to borrow the $100.

Implications of Non-payment: Some lenders in our network may automatically roll over your existing loan for another two weeks if you don't pay back the loan on time. Fees for renewing the loan range from lender to lender. Most of the time these fees equal the fees you paid to get the initial payday loan. We ask lenders in our network to follow legal and ethical collection practices set by industry associations and government agencies. Non-payment of a payday loan might negatively effect your credit history.

Calculate APR

Happily Served Customers

Get pre-approved for a large personal loan today!