SCREENING METHODS: Gradient plates

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A method similar to the agar dilution assay is the gradient plate technique. In this method, melted agar is dispensed into a Petri dish with one edge elevated. The agar is allowed to solidify and form a wedge. A second, overlay wedge which contains the antimicrobial is then poured. The result is a plate which […]

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SCREENING METHODS: Broth dilution methods(5)

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The agar dilution method was used to determine the strength of the antimicrobial activity of medicinal plants extract, spices, and essential oils by Banerjee et al, (1982), Kivanc and Akguel (1986), Bara and Vanetti (1995), Sekiyama et al. (1996), Firouzi et al. (1998), Ward et al. (1998), Hammer et al. (1999), Elgayyar et al. (2001), […]

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SCREENING METHODS: Broth dilution methods(4)

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In some cases, the minimum bactericidal concentration (MBC) or the bacteriostatic concentration is stated, both terms agreeing closely with the MIC. A list of the most frequently used terms in antimicrobial activity testing of crude medicinal plants extracts and essential oils are presented in Table-1.

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SCREENING METHODS: Broth dilution methods(3)

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While these micro-broth methods generally work well for plant extracts, problems arise when the extract is heavily colored as this can interfere with the measurement of the indicator chemical. Further, as these methods use plastic microtiter plates, essential oils that have a solvent action on plastics (e.g. Letospermum petersonii, Backhousia citriodora) cannot be used. Also […]

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SCREENING METHODS: Broth dilution methods(2)

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For organisms sensitive to oxygen tension in the media this can present an additional problem as the oil can inhibit gaseous exchange. Tween or ethanol may be used to enhance incorporation into the aqueous media, however as previously discussed these compounds may interfere with the assay results. The broth assay was used in both the […]

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SCREENING METHODS: Broth dilution methods(1)

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Difficulties with partitioning of hydrophobic compounds in agar a more accurate method was developed known as broth dilution method. In this method, bacteria are grown in test-tubes in a liquid media in the presence of the test substance (plate-3) (Panda et al., 2009a,b,c). At regular time intervals (e.g. every 10 min or every hour) a […]

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SCREENING METHODS: Agar cup method (Cont.)

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During the evaluation of antimicrobial activity of medicinal plant extracts by agar dilution method, several researchers used different solvents (Prudent et al, 1995; Pintore et al., 2002), different volumes of inoculums (Juven et al., 1994; Prudent et al., 1995), various inoculation techniques, e.g. dotting (Pintore et al, 2002) or streaking (Farag et al, 1989). Despite […]

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