SCREENING METHODS: Agar cup method

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The principle of the agar cup or agar well diffusion is the same as that of the agar disk diffusion method. A standardized inoculum culture is spread evenly on the surface of gelled gar plates. Wells of between 6 and 8 mm are aseptically punched on the agar using a sterile cork borer allowing at […]

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SCREENING METHODS: Disk diffusion method(2)

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A paper disk (6 or 8 mm) containing a known volume of the test substance is then placed in the center of the agar and the dish incubated for 24 h or more. At this time the “cleared” zone (zone of inhibition) surrounding the disk is measured and compared with zones for standard antibiotics or […]

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SCREENING METHODS: Disk diffusion method(1)

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Factors such as the volume of extract placed on the paper disk, the thickness of the agar layer and whether a solvent is used vary considerable between studies. On the other hand in agar well test is a quantitative method in which the extract is deposited into well cut into the agar and can be […]

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SCREENING METHODS: Agar Diffusion

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The compound diffuses through the agar, setting up a concentration gradient. The concentration is inversely proportional to the distance from disk or area. Inhibition, which is the measure of activity, is indicated by a zone without growth of the organism around the disk or well. The size of the zone is dependent on the rate […]

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SCREENING METHODS: Antibacterial assays

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Methods which are used to evaluate the activity of plant antimicrobial are divided into in vitro and in vivo (application test). The former may be termed “screening methods” and might include any test in which the compound is not applied directly to the product under use conditions. Generally, these tests provide preliminary information to determined […]

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SCREENING METHODS: TESTING

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Antimicrobial susceptibility test (AST) is used to determine the efficacy of potential antimicrobials from natural products against a number of microorganisms. In clinical research, in vitro susceptibility tests are important if an organism is suspected toward resistance to frequently used antimicrobial agents. Today, numerous standard methods, approved by different organization like the National Committee for […]

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Brand positioning in financial services: LIMITATIONS AND FUTURE RESEARCH

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A key limitation of this research is that the measurement of brand-attribute association did not include an individual ‘strength’ indicator. In this research an attribute was either associated with the brand or not. This technique was used because of its popularity in commercial market research and use to identify brand positions, via perceptual mapping techniques. […]

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